The adjuvant effects of UA did not require the inflammasome (NIrp3, Pycard) or the interleukin-1 (Myd88, IL-1r) axis. UA crystals promoted Th2 cell immunity by activating dendritic cells through spleen tyrosine kinase and PI3-kinase delta signaling. These findings provide further molecular insight into Th2 cell development and identify UA as an essential initiator and amplifier of allergic inflammation.”
“In inflamed venules, neutrophils roll on P- or E-selectin, engage P-selectin glycoprotein ligand-1 (PSGL-1), and signal extension of integrin alpha(L)beta(2) in a low affinity state to slow rolling on intercellular adhesion molecule-1 (ICAM-1). Cytoskeleton-dependent

receptor clustering often triggers signaling, and it has been hypothesized that the cytoplasmic domain links PSGL-1

Selleckchem JQ1 to the cytoskeleton. Chemokines cause rolling neutrophils to fully activate alpha(L)beta(2), leading to arrest on ICAM-1. Cytoskeletal anchorage of alpha(L)beta(2) has been linked to chemokine-triggered extension and force-regulated conversion to the high affinity Nirogacestat state. We asked whether PSGL-1 must interact with the cytoskeleton to initiate signaling and whether alpha(L)beta(2) must interact with the cytoskeleton to extend. Fluorescence recovery after photobleaching of transfected cells documented cytoskeletal restraint of PSGL-1. The lateral mobility of PSGL-1 similarly increased by depolymerizing actin filaments with latrunculin B or by mutating the cytoplasmic tail to impair binding to the cytoskeleton. Converting dimeric PSGL-1 to a monomer by replacing its transmembrane domain did not alter its mobility. By transducing retroviruses expressing

WT or mutant PSGL-1 into bone marrow-derived macrophages from PSGL-1-deficient mice, we show that PSGL-1 required neither dimerization nor cytoskeletal anchorage to signal beta(2) integrin-dependent slow rolling on P- selectin and ICAM-1. Depolymerizing actin filaments or decreasing actomyosin tension in neutrophils did not impair PSGL-1- or chemokine-mediated integrin extension. Unlike chemokines, PSGL-1 did not signal cytoskeleton-dependent swing out of the beta(2)-hybrid domain associated with the high affinity state. The cytoskeletal independence of PSGL-1- initiated, alpha(L)beta(2)-mediated slow rolling SB203580 chemical structure differs markedly from the cytoskeletal dependence of chemokine-initiated, alpha(L)beta(2)-mediated arrest.”
“Perfusion imaging is crucial in imaging of ischemic stroke to determine ’tissue at risk’ for infarction. In this study we compared the volumetric quantification of the perfusion deficit in two rat middle-cerebral-artery occlusion (MCAO) models using two gadolinium-based contrast agents (P1152 (Guerbet) and Magnevist (Bayer-Schering, Pittsburgh, PA, USA)) as compared with our well established continuous arterial spin labeling (CASL) perfusion imaging technique.